human PDE5A shRNA silencing AAV

Name
AAV-h-PDE5A-shRNA

Cat No
shAAV-214408

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PDE5A.

shAAV-214408

AAV-h-PDE5A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PDE5A (phosphodiesterase 5A). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CGB-PDE; CN5A; PDE5

Description (Vector)

PDE5A encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Gene ID

8654

Gene Name (Vector)

phosphodiesterase 5A

Gene Symbol

PDE5A

HGNC ID

HGNC:8784

ORF Size (aa)

2628

ORF Size (bp)

2628 bp

RefSeq ID

NM_001083

RefSeq Synonyms

NM_033437, NM_033430, NM_001083, BC126233,

Species

human

UniGene ID

Hs.587281

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.