human LIMK1 shRNA silencing AAV

Name 
AAV-h-LIMK1-shRNA
Cat No 
shAAV-213950
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human LIMK1.

shAAV-213950
AAV-h-LIMK1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human LIMK1 (LIM domain kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1
Description (eCommerce)
LIM domain kinase 1 (LIMK1) is a serine/threonine protein kinase that bears 2 LIM zinc-binding domains. LIMK1 participates in the regulation of actin filament dynamics and may play a role in synapse formation and function.
Description (Vector)
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
3984
Gene Name (eCommerce)
LIM domain kinase 1
Gene Name (Vector)
LIM domain kinase 1
Gene Symbol
LIMK1
HGNC ID
HGNC:6613
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1944
ORF Size (bp)
1944 bp
Protein Name (eCommerce)
LIM domain kinase 1
RefSeq ID
NM_002314
RefSeq Synonyms
NM_002314, NM_001204426, BC152982, BC148340,
Research Areas
Apoptosis,Axon Guidance,Cytoskeleton,Immunology,Innate Immunity,Kinase/Phosphatase,Leptin Signaling,Neurobiology,Neurodevelopment,RAC1 Signaling,Rho Signaling,Signal Transduction
Research Areas (Faceted)
cell_biology,developmental_biology,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
LIM domain kinase 1 (LIMK1) is a serine/threonine protein kinase that bears 2 LIM zinc-binding domains. LIMK1 participates in the regulation of actin filament dynamics and may play a role in synapse formation and function.
UniGene ID
Hs.647035
UniProt ID (eCommerce)
P53667

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.