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human ILF3 shRNA silencing AAV

Name 
AAV-h-ILF3-shRNA
Cat No 
shAAV-212225
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ILF3.

shAAV-212225
AAV-h-ILF3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ILF3 (interleukin enhancer binding factor 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CBTF; Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBF; DRBP76; dsRNA binding protein NFAR-2/MPP4; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; interleukin enhancer-binding factor 3; MMP4; M-phase phosphoprotein 4; MPHOSPH4; MPP4; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1; NFAR2; NF-AT-90; nuclear factor associated with dsRNA; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; translational control protein 80
Description (eCommerce)
NFAR (nuclear factor associated with dsRNA) exists as two major protein species of 90 kDa (NFAR-1 or NF90) and 110 kDa (NFAR-2 or NF110) in the cell. NF90 and NF110 arise from a single gene named interleukin enhancer binding factor (ILF3) gene which resides on chromosome 19p13 and are generated through alternative splicing events. NF90 is a dsRNA binding protein that stabilizes mRNAs and regulates transcription. NF90 is a subunit of the nuclear factor of activated T-cells (NFAT) transcription factor that is required for T-cell expression of interleukin 2. The NF110 isoform is restricted to the nucleus and its function may be unrelated to NF90.
Description (Vector)
ILF3 encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
3609
Gene Name (eCommerce)
interleukin enhancer binding factor 3
Gene Name (Vector)
interleukin enhancer binding factor 3
Gene Symbol
ILF3
HGNC ID
HGNC:6038
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2121
ORF Size (bp)
2121 bp
Protein Name (eCommerce)
Interleukin enhancer-binding factor 3
RefSeq ID
NM_004516
RefSeq Synonyms
NM_153464, NM_017620, NM_012218, NM_004516, NM_001137673, BC064836, BC003086,
Research Areas
Cell Cycle,Host-Virus Interactions,Mitochondrion,RNA Binding,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,infectious_disease,transcription_translation
Species
human
Target Sentence
NFAR (nuclear factor associated with dsRNA) exists as two major protein species of 90 kDa (NFAR-1 or NF90) and 110 kDa (NFAR-2 or NF110) in the cell. NF90 and NF110 arise from a single gene named interleukin enhancer binding factor (ILF3) gene which resides on chromosome 19p13 and are generated through alternative splicing events. NF90 is a dsRNA binding protein that stabilizes mRNAs and regulates transcription. NF90 is a subunit of the nuclear factor of activated T-cells (NFAT) transcription factor that is required for T-cell expression of interleukin 2. The NF110 isoform is restricted to the nucleus and its function may be unrelated to NF90.
UniGene ID
Hs.465885
UniProt ID (eCommerce)
Q12906

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.