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human HTT shRNA silencing AAV

Name 
AAV-h-HTT-shRNA
Cat No 
shAAV-211718
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human HTT.

shAAV-211718
AAV-h-HTT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HTT (huntingtin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HD; HD protein; huntingtin; huntington disease protein; IT15; LOMARS
Description (eCommerce)
The Huntingtin (HTT) gene is linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene product is widely expressed and is required for normal development [taken from NCBI Entrez Gene (Gene ID: 3064)].
Description (Vector)
Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.
Gene ID
3064
Gene Name (eCommerce)
huntingtin
Gene Name (Vector)
huntingtin
Gene Symbol
HTT
HGNC ID
HGNC:4851
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
9435
ORF Size (bp)
9435 bp
Protein Name (eCommerce)
Huntingtin
RefSeq ID
NM_002111
RefSeq Synonyms
NM_002111, BC172756,
Research Areas
Aging,Apoptosis,Autophagy,Cell Cycle,EGFR Signaling,Golgi Apparatus,Huntington's Disease,Neurobiology,Neurodegeneration,Neurodevelopment,Signal Transduction,Spermatogenesis,Transcription Factor/Regulator,Transport
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
The Huntingtin (HTT) gene is linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene product is widely expressed and is required for normal development [taken from NCBI Entrez Gene (Gene ID: 3064)].
UniGene ID
Hs.518450
UniProt ID (eCommerce)
P42858

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.