human HSD11B2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human HSD11B2.
shAAV-211559
AAV-h-HSD11B2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human HSD11B2 (hydroxysteroid 11-beta dehydrogenase 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AME; AME1; HSD11K; HSD2; SDR9C3
Description (Vector)
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.
Gene ID
3291
Gene Name (Vector)
hydroxysteroid 11-beta dehydrogenase 2
Gene Symbol
HSD11B2
HGNC ID
HGNC:5209
ORF Size (aa)
1218
RefSeq ID
NM_000196
RefSeq Synonyms
NM_000196, BC064536, BC036780,
Species
human
UniGene ID
Hs.1376
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.