human HPRT1 shRNA silencing AAV

Name
AAV-h-HPRT1-shRNA

Cat No
shAAV-211501

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human HPRT1.

shAAV-211501

AAV-h-HPRT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HPRT1 (hypoxanthine phosphoribosyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HGPRT; HGPRTase; HPRT; hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89

Description (eCommerce)

HPRT1 is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway [taken from NCBI Entrez Gene (Gene ID: 3251)].

Description (Vector)

HPRT1 is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Gene ID

3251

Gene Name (eCommerce)

hypoxanthine phosphoribosyltransferase 1

Gene Name (Vector)

hypoxanthine phosphoribosyltransferase 1

Gene Symbol

HPRT1

HGNC ID

HGNC:5157

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

657

Protein Name (eCommerce)

Hypoxanthine-guanine phosphoribosyltransferase

RefSeq ID

NM_000194

RefSeq Synonyms

NM_000194, BC000578,

Research Areas

Immunology,Metabolism/Metabolic Process,Neurobiology,Neurodevelopment,Spermatogenesis

Research Areas (Faceted)

cell_biology,developmental_biology,immunology,metabolism,neurobiology

Species

human

Target Sentence

HPRT1 is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway [taken from NCBI Entrez Gene (Gene ID: 3251)].

UniGene ID

Hs.412707

UniProt ID (eCommerce)

P00492

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.