human HMGCL shRNA silencing AAV

Name
AAV-h-HMGCL-shRNA

Cat No
shAAV-211281

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human HMGCL.

shAAV-211281

AAV-h-HMGCL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HMGCL (3-hydroxymethyl-3-methylglutaryl-CoA lyase). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Description (Vector)

HMGCL belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

3155

Gene Name (Vector)

3-hydroxymethyl-3-methylglutaryl-CoA lyase

Gene Symbol

HMGCL

HGNC ID

HGNC:5005

ORF Size (aa)

978

ORF Size (bp)

978 bp

RefSeq ID

NM_000191

RefSeq Synonyms

NM_001166059, NM_000191, BC010570,

Species

human

UniGene ID

Hs.533444

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.