human GNPTAB shRNA silencing AAV

Name
AAV-h-GNPTAB-shRNA

Cat No
shAAV-210138

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human GNPTAB.

shAAV-210138

AAV-h-GNPTAB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GNPTAB (N-acetylglucosamine-1-phosphate transferase alpha and beta subunits). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

GNPTA; ICD

Description (Vector)

GNPTAB encodes two of three subunit types of the membrane-bound enzyme N-acetylglucosamine-1-phosphotransferase, a heterohexameric complex composed of two alpha, two beta, and two gamma subunits. The encoded protein is proteolytically cleaved at the Lys928-Asp929 bond to yield mature alpha and beta polypeptides while the gamma subunits are the product of a distinct gene (GeneID 84572). In the Golgi apparatus, the heterohexameric complex catalyzes the first step in the synthesis of mannose 6-phosphate recognition markers on certain oligosaccharides of newly synthesized lysosomal enzymes. These recognition markers are essential for appropriate trafficking of lysosomal enzymes. Mutations in this gene have been associated with both mucolipidosis II and mucolipidosis IIIA.

Gene ID

79158

Gene Name (Vector)

N-acetylglucosamine-1-phosphate transferase alpha and beta subunits

Gene Symbol

GNPTAB

HGNC ID

HGNC:29670

ORF Size (aa)

3771

ORF Size (bp)

3771 bp

RefSeq ID

NM_024312

RefSeq Synonyms

NM_024312, BC131787, BC002779,

Species

human

UniGene ID

Hs.46850

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.