human GLCE shRNA silencing AAV

Name
AAV-h-GLCE-shRNA

Cat No
shAAV-209959

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human GLCE.

shAAV-209959

AAV-h-GLCE-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GLCE (glucuronic acid epimerase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HSEPI

Description (Vector)

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 (PubMed 15853773)).(supplied by OMIM, Jun 2008)

Gene ID

26035

Gene Name (Vector)

glucuronic acid epimerase

Gene Symbol

GLCE

HGNC ID

HGNC:17855

ORF Size (aa)

1854

ORF Size (bp)

1854 bp

RefSeq ID

NM_015554

RefSeq Synonyms

NM_015554, NM_001324094, NM_001324093, NM_001324092, NM_001324091, BC160046,

Species

human

UniGene ID

Hs.183006

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.