human GCA shRNA silencing AAV

Name 
AAV-h-GCA-shRNA
Cat No 
shAAV-209728
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GCA.

shAAV-209728
AAV-h-GCA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GCA (grancalcin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
GCL
Description (Vector)
GCA product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.
Gene ID
25801
Gene Name (Vector)
grancalcin
Gene Symbol
GCA
HGNC ID
HGNC:15990
ORF Size (aa)
654
ORF Size (bp)
654 bp
RefSeq ID
NM_012198
RefSeq Synonyms
NM_012198, NM_001330271, NM_001330270, NM_001330268, NM_001330267, NM_001330266, NM_001330265, BC005214,
Species
human
UniGene ID
Hs.377894

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.