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human GAK shRNA silencing AAV

Name 
AAV-h-GAK-shRNA
Cat No 
shAAV-209562
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GAK.

shAAV-209562
AAV-h-GAK-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GAK (cyclin G associated kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
auxilin-2; cyclin-G-associated kinase; DNAJ26; DNAJC26
Description (eCommerce)
Cyclin-G-associated Kinase (GAK) associates with cyclin G and CDK5. GAK seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. GAK expression oscillates slightly during the cell cycle, peaking at G1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O14976].
Description (Vector)
In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5.
Gene ID
2580
Gene Name (eCommerce)
cyclin G associated kinase
Gene Name (Vector)
cyclin G associated kinase
Gene Symbol
GAK
HGNC ID
HGNC:4113
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3699
ORF Size (bp)
3699 bp
Protein Name (eCommerce)
Cyclin-G-associated kinase
RefSeq ID
NM_005255
RefSeq Synonyms
NM_005255, NM_001318134, BC085005,
Research Areas
Cell Cycle,Golgi Apparatus,Immunology,Kinase/Phosphatase
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,immunology,signal_transduction
Species
human
Target Sentence
Cyclin-G-associated Kinase (GAK) associates with cyclin G and CDK5. GAK seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. GAK expression oscillates slightly during the cell cycle, peaking at G1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O14976].
UniGene ID
Hs.369607
UniProt ID (eCommerce)
O14976

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.