human FEN1 shRNA silencing AAV

Name 
AAV-h-FEN1-shRNA
Cat No 
shAAV-208900
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human FEN1.

shAAV-208900
AAV-h-FEN1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human FEN1 (flap structure-specific endonuclease 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DNase IV; FEN-1; flap endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; Maturation factor 1; maturation factor-1; MF1; RAD2
Description (eCommerce)
Flap endonuclease 1 (Fen1) is a member of the XPG/RAD2 endonuclease family. Fen1 functions primarily as an endonuclease that removes 5’-overhangs during base-excision DNA repair and lagging strand DNA synthesis. For these processes Fen1 interacts with PCNA and the 9-1-1 DNA repair complex. Fen1 also possesses 5’ to 3’ exonuclease activity as well as RNase H activity.
Description (Vector)
FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Gene ID
2237
Gene Name (eCommerce)
flap structure-specific endonuclease 1
Gene Name (Vector)
flap structure-specific endonuclease 1
Gene Symbol
FEN1
HGNC ID
HGNC:3650
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1143
ORF Size (bp)
1143 bp
Protein Name (eCommerce)
Flap endonuclease 1
RefSeq ID
NM_004111
RefSeq Synonyms
NM_004111, BC000323,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Host-Virus Interactions,Mitochondrion,Signal Transduction,Telomeric Maintenance
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,infectious_disease,signal_transduction,transcription_translation
Species
human
Target Sentence
Flap endonuclease 1 (Fen1) is a member of the XPG/RAD2 endonuclease family. Fen1 functions primarily as an endonuclease that removes 5’-overhangs during base-excision DNA repair and lagging strand DNA synthesis. For these processes Fen1 interacts with PCNA and the 9-1-1 DNA repair complex. Fen1 also possesses 5’ to 3’ exonuclease activity as well as RNase H activity.
UniGene ID
Hs.409065
UniProt ID (eCommerce)
P39748

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.