human ESPL1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human ESPL1.
shAAV-208110
AAV-h-ESPL1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human ESPL1 (extra spindle pole bodies like 1, separase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
ESP1; SEPA
Description (Vector)
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 (PubMed 19345191)).(supplied by OMIM, Nov 2010)
Gene ID
9700
Gene Name (Vector)
extra spindle pole bodies like 1, separase
Gene Symbol
ESPL1
HGNC ID
HGNC:16856
ORF Size (aa)
6363
ORF Size (bp)
6363 bp
RefSeq ID
NM_012291
RefSeq Synonyms
NM_012291, BC172570, BC172238,
Species
human
UniGene ID
Hs.153479
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.