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human EIF5B shRNA silencing AAV

Name 
AAV-h-EIF5B-shRNA
Cat No 
shAAV-207809
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human EIF5B.

shAAV-207809
AAV-h-EIF5B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human EIF5B (eukaryotic translation initiation factor 5B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
eIF-5B; eukaryotic translation initiation factor 5B; IF2; translation initiation factor IF2; translation initiation factor IF-2
Description (eCommerce)
Eukaryotic initiation factor 5 subunit B (eIF5B) is the eukaryotic ortholog of the bacterial IF2 initiation factor. It is one of two essential GTPases required for translation initiation. eIF5B plays a role in facilitiating 60S and 40S ribosomal subunit joining to form the 80S ribosome in the final step of translation.
Description (Vector)
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Gene ID
9669
Gene Name (eCommerce)
eukaryotic translation initiation factor 5B
Gene Name (Vector)
eukaryotic translation initiation factor 5B
Gene Symbol
EIF5B
HGNC ID
HGNC:30793
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3663
ORF Size (bp)
3663 bp
Protein Name (eCommerce)
Eukaryotic translation initiation factor 5B
RefSeq ID
NM_015904
RefSeq Synonyms
NM_015904, BC032639,
Research Areas
Gene Expression,Metabolism/Metabolic Process,RNA Transport,Translational Control
Research Areas (Faceted)
genetics,transcription_translation,metabolism,cell_biology
Species
human
Target Sentence
Eukaryotic initiation factor 5 subunit B (eIF5B) is the eukaryotic ortholog of the bacterial IF2 initiation factor. It is one of two essential GTPases required for translation initiation. eIF5B plays a role in facilitiating 60S and 40S ribosomal subunit joining to form the 80S ribosome in the final step of translation.
UniGene ID
Hs.158688
UniProt ID (eCommerce)
O60841

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.