human E2F2 shRNA silencing AAV

Name
AAV-h-E2F2-shRNA

Cat No
shAAV-207492

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human E2F2.

shAAV-207492

AAV-h-E2F2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human E2F2 (E2F transcription factor 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

E2F-2

Description (Vector)

E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.

Gene ID

1870

Gene Name (Vector)

E2F transcription factor 2

Gene Symbol

E2F2

HGNC ID

HGNC:3114

ORF Size (aa)

1314

ORF Size (bp)

1314 bp

RefSeq ID

NM_004091

RefSeq Synonyms

NM_004091, BC053676, BC007609,

Species

human

UniGene ID

Hs.194333

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.