human DLD shRNA silencing AAV

Name 
AAV-h-DLD-shRNA
Cat No 
shAAV-207034
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DLD.

shAAV-207034
AAV-h-DLD-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DLD (dihydrolipoamide dehydrogenase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
diaphorase; Dihydrolipoamide dehydrogenase; dihydrolipoyl dehydrogenase, mitochondrial; DLDD; DLDH; E3; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; epididymis secretory sperm binding protein; GCSL; glycine cleavage system L protein; glycine cleavage system protein L; LAD; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; PHE3
Description (eCommerce)
Dihydrolipoamide dehydrogenase (DLD) is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. DLD has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, DLD functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency [taken from NCBI Entrez Gene (Gene ID: 1738)].
Description (Vector)
DLD encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
Gene ID
1738
Gene Name (eCommerce)
dihydrolipoamide dehydrogenase
Gene Name (Vector)
dihydrolipoamide dehydrogenase
Gene Symbol
DLD
HGNC ID
HGNC:2898
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1530
ORF Size (bp)
1530 bp
Protein Name (eCommerce)
Dihydrolipoyl dehydrogenase, mitochondrial
RefSeq ID
NM_000108
RefSeq Synonyms
NM_001289752, NM_001289751, NM_001289750, NM_000108, BC018696, BC018648,
Research Areas
Aging,Metabolism/Metabolic Process,Mitochondrion
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
Dihydrolipoamide dehydrogenase (DLD) is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. DLD has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, DLD functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency [taken from NCBI Entrez Gene (Gene ID: 1738)].
UniGene ID
Hs.131711
UniProt ID (eCommerce)
P09622

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.