human DLAT shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human DLAT.
shAAV-207032
AAV-h-DLAT-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human DLAT (dihydrolipoamide S-acetyltransferase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
DLTA; PDC-E2; PDCE2
Description (Vector)
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Gene ID
1737
Gene Name (Vector)
dihydrolipoamide S-acetyltransferase
Gene Symbol
DLAT
HGNC ID
HGNC:2896
ORF Size (aa)
1944
ORF Size (bp)
1944 bp
RefSeq ID
NM_001931
RefSeq Synonyms
NM_001931, BC039084,
Species
human
UniGene ID
Hs.335551
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.