human DDX6 shRNA silencing AAV

Name 
AAV-h-DDX6-shRNA
Cat No 
shAAV-206777
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DDX6.

shAAV-206777
AAV-h-DDX6-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DDX6 (DEAD-box helicase 6). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); HLR2; oncogene RCK; P54; probable ATP-dependent RNA helicase DDX6; RCK
Description (eCommerce)
DDX6 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX6 has been observed to positively regulate growth by modulating gene expression at the translational level.
Description (Vector)
DDX6 encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Gene ID
1656
Gene Name (eCommerce)
DEAD-box helicase 6
Gene Name (Vector)
DEAD-box helicase 6
Gene Symbol
DDX6
HGNC ID
HGNC:2747
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1452
ORF Size (bp)
1452 bp
Protein Name (eCommerce)
Probable ATP-dependent RNA helicase DDX6
RefSeq ID
NM_004397
RefSeq Synonyms
NM_004397, NM_001257191, BC065007,
Research Areas
Cancer,EGFR Signaling,Gene Expression,Helicase Activity,RNA Binding,RNA Processing,Signal Transduction
Research Areas (Faceted)
cancer,signal_transduction,genetics,transcription_translation,cell_biology,genetics
Species
human
Target Sentence
DDX6 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX6 has been observed to positively regulate growth by modulating gene expression at the translational level.
UniGene ID
Hs.654366
UniProt ID (eCommerce)
P26196

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.