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human DDX23 shRNA silencing AAV

Name 
AAV-h-DDX23-shRNA
Cat No 
shAAV-206742
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DDX23.

shAAV-206742
AAV-h-DDX23-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DDX23 (DEAD-box helicase 23). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
100 kDa U5 snRNP-specific protein; DEAD (Asp-Glu-Ala-Asp) box polypeptide 23; DEAD box protein 23; probable ATP-dependent RNA helicase DDX23; prp28; PRP28 homolog; PRP28 homolog, yeast; PRP28p homolog; PRPF28; SNRNP100; U5 snRNP 100 kD protein; U5-100K; U5-100KD
Description (eCommerce)
DDX23 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX23 is a component of the U5 snRNP that may facilitate conformational changes in the splicesome during pre-mRNA splicing.
Description (Vector)
DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.
Gene ID
9416
Gene Name (eCommerce)
DEAD-box helicase 23
Gene Name (Vector)
DEAD-box helicase 23
Gene Symbol
DDX23
HGNC ID
HGNC:17347
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2463
ORF Size (bp)
2463 bp
Protein Name (eCommerce)
Probable ATP-dependent RNA helicase DDX23
RefSeq ID
NM_004818
RefSeq Synonyms
NM_004818, BC002366,
Research Areas
Gene Expression,Helicase Activity,Mitochondrion,mRNA Processing,mRNA Splicing,RNA Processing,Spliceosome
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
DDX23 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX23 is a component of the U5 snRNP that may facilitate conformational changes in the splicesome during pre-mRNA splicing.
UniGene ID
Hs.130098
UniProt ID (eCommerce)
Q9BUQ8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.