human CYP27B1 shRNA silencing AAV

Name
AAV-h-CYP27B1-shRNA

Cat No
shAAV-206470

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CYP27B1.

shAAV-206470

AAV-h-CYP27B1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CYP27B1 (cytochrome P450 family 27 subfamily B member 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR

Description (Vector)

CYP27B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Gene ID

1594

Gene Name (Vector)

cytochrome P450 family 27 subfamily B member 1

Gene Symbol

CYP27B1

HGNC ID

HGNC:2606

ORF Size (aa)

1527

ORF Size (bp)

1527 bp

RefSeq ID

NM_000785

RefSeq Synonyms

NM_000785, BC136386, BC001776,

Species

human

UniGene ID

Hs.524528

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.