human CRB1 shRNA silencing AAV

Name
AAV-h-CRB1-shRNA

Cat No
shAAV-205963

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CRB1.

shAAV-205963

AAV-h-CRB1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CRB1 (crumbs 1, cell polarity complex component). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

LCA8; RP12

Description (Vector)

CRB1 encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.

Gene ID

23418

Gene Name (Vector)

crumbs 1, cell polarity complex component

Gene Symbol

CRB1

HGNC ID

HGNC:2343

ORF Size (aa)

4221

ORF Size (bp)

4221 bp

RefSeq ID

NM_201253

RefSeq Synonyms

NM_201253, NM_001257966, NM_001257965, NM_001193640, BC136271,

Species

human

UniGene ID

Hs.126135

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.