human COL4A3 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human COL4A3.
shAAV-205706
AAV-h-COL4A3-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human COL4A3 (collagen type IV alpha 3 chain). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Gene ID
1285
Gene Name (Vector)
collagen type IV alpha 3 chain
Gene Symbol
COL4A3
HGNC ID
HGNC:2204
ORF Size (aa)
5010
ORF Size (bp)
5010 bp
RefSeq ID
NM_000091
RefSeq Synonyms
NM_000091, BC156138,
Species
human
UniGene ID
Hs.570065
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.