human COL2A1 shRNA silencing AAV

Name
AAV-h-COL2A1-shRNA

Cat No
shAAV-205703

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human COL2A1.

shAAV-205703

AAV-h-COL2A1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human COL2A1 (collagen type II alpha 1 chain). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ANFH; AOM; COL11A3; SEDC; STL1

Description (Vector)

COL2A1 encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Gene ID

1280

Gene Name (Vector)

collagen type II alpha 1 chain

Gene Symbol

COL2A1

HGNC ID

HGNC:2200

ORF Size (aa)

4464

ORF Size (bp)

4464 bp

RefSeq ID

NM_001844

RefSeq Synonyms

NM_033150, NM_001844, BC116449, BC007252,

Species

human

UniGene ID

Hs.408182

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.