human COL1A2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human COL1A2.
shAAV-205690
AAV-h-COL1A2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human COL1A2 (collagen type I alpha 2 chain). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
OI4
Description (Vector)
COL1A2 encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. (provided by R. Dalgleish, Feb 2008)
Gene ID
1278
Gene Name (Vector)
collagen type I alpha 2 chain
Gene Symbol
COL1A2
HGNC ID
HGNC:2198
ORF Size (aa)
4101
ORF Size (bp)
4101 bp
RefSeq ID
NM_000089
RefSeq Synonyms
NM_000089, BC054498, BC042586,
Species
human
UniGene ID
Hs.489142
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.