human CHST14 shRNA silencing AAV

Name
AAV-h-CHST14-shRNA

Cat No
shAAV-205243

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CHST14.

shAAV-205243

AAV-h-CHST14-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CHST14 (carbohydrate sulfotransferase 14). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ATCS; D4ST1; EDSMC1; HNK1ST

Description (Vector)

CHST14 encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.

Gene ID

113189

Gene Name (Vector)

carbohydrate sulfotransferase 14

Gene Symbol

CHST14

HGNC ID

HGNC:24464

ORF Size (aa)

1131

ORF Size (bp)

1131 bp

RefSeq ID

NM_130468

RefSeq Synonyms

NM_130468, BC053633, BC049214,

Species

human

UniGene ID

Hs.442449

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.