human CHRNB3 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human CHRNB3.
shAAV-205224
AAV-h-CHRNB3-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human CHRNB3 (cholinergic receptor nicotinic beta 3 subunit). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 (PubMed 9009220)). Elliott et al. (1996) (PubMed 8906617) stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.(supplied by OMIM, Apr 2010)
Gene ID
1142
Gene Name (Vector)
cholinergic receptor nicotinic beta 3 subunit
Gene Symbol
CHRNB3
HGNC ID
HGNC:1963
ORF Size (aa)
1377
ORF Size (bp)
1377 bp
RefSeq ID
NM_000749
RefSeq Synonyms
NM_000749, BC069788, BC069703, BC069681,
Species
human
UniGene ID
Hs.654576
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.