human CCNE1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human CCNE1.
shAAV-204495
AAV-h-CCNE1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human CCNE1 (cyclin E1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CCNE; G1/S-specific cyclin-E1; pCCNE1
Description (eCommerce)
Cyclin E1 is a regulatory subunit of the CDK2 kinase. Cyclin E is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis.
Description (Vector)
CCNE1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
Gene ID
898
Gene Name (eCommerce)
cyclin E1
Gene Name (Vector)
cyclin E1
Gene Symbol
CCNE1
HGNC ID
HGNC:1589
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1233
ORF Size (bp)
1233 bp
Protein Name (eCommerce)
G1/S-specific cyclin-E1
RefSeq ID
NM_001238
RefSeq Synonyms
NM_001322262, NM_001322261, NM_001322259, NM_001238, BC035498,
Research Areas
Cancer,Cell Cycle,Cell Division,DNA Damage/Repair,DNA Replication,Glioma,Host-Virus Interactions,Immunology,Interleukin Signaling,Lung Cancer,Meiosis,MicroRNAs in Cancer,mTOR Signaling,Neurobiology,Neurodegeneration,p53 Signaling,Parkinson's Disease,Parkin-Ubiquitin Proteasomal System,PI3K/Akt Signaling,Prostate Cancer,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,TSH Signaling,Viral Carcinogenesis,Wnt Signaling
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Cyclin E1 is a regulatory subunit of the CDK2 kinase. Cyclin E is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis.
UniGene ID
Hs.244723
UniProt ID (eCommerce)
P24864
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.