human BRAP shRNA silencing AAV

Name 
AAV-h-BRAP-shRNA
Cat No 
shAAV-202249
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human BRAP.

shAAV-202249
AAV-h-BRAP-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BRAP (BRCA1 associated protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
BRAP2; BRCA1-associated protein; galectin-2-binding protein; IMP; impedes mitogenic signal propagation; renal carcinoma antigen NY-REN-63; RING finger protein 52; RING-type E3 ubiquitin transferase BRAP2; RNF52
Description (eCommerce)
BRCA1-associated protein (BRAP) negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. BRAP acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. BRAP may also act as a cytoplasmic retention protein with a role in regulating nuclear transport [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z569].
Description (Vector)
BRAP was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm.
Gene ID
8315
Gene Name (eCommerce)
BRCA1 associated protein
Gene Name (Vector)
BRCA1 associated protein
Gene Symbol
BRAP
HGNC ID
HGNC:1099
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1779
ORF Size (bp)
1779 bp
Protein Name (eCommerce)
BRCA1-associated protein
RefSeq ID
NM_006768
RefSeq Synonyms
NM_006768, BC136699, BC136698,
Research Areas
MAPK Signaling,Signal Transduction,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cell_biology,signal_transduction
Species
human
Target Sentence
BRCA1-associated protein (BRAP) negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. BRAP acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. BRAP may also act as a cytoplasmic retention protein with a role in regulating nuclear transport [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z569].
UniGene ID
Hs.530940
UniProt ID (eCommerce)
Q7Z569

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.