human BHLHE22 shRNA silencing AAV

Name 
AAV-h-BHLHE22-shRNA
Cat No 
shAAV-202127
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human BHLHE22.

shAAV-202127
AAV-h-BHLHE22-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BHLHE22 (basic helix-loop-helix family member e22). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Beta3; Beta3a; BHLHB5; CAGL85; TNRC20
Description (Vector)
BHLHE22 belongs to the basic helix-loop-helix (bHLH) family of transcription factors that regulate cell fate determination, proliferation, and differentiation. These proteins function as dimers and bind to an E-box DNA sequence (CANNTG). BHLHE22 is expressed exclusively in the central nervous system and retina (Xu et al., 2002 (PubMed 12213201)).(supplied by OMIM, Jul 2010)
Gene ID
27319
Gene Name (Vector)
basic helix-loop-helix family member e22
Gene Symbol
BHLHE22
HGNC ID
HGNC:11963
ORF Size (aa)
1146
ORF Size (bp)
1146 bp
RefSeq ID
NM_152414
RefSeq Synonyms
NM_152414, BC156671,
Species
human
UniGene ID
Hs.591870

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.