human ATP5J shRNA silencing AAV

Name
AAV-h-ATP5J-shRNA

Cat No
shAAV-201729

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ATP5J.

shAAV-201729

AAV-h-ATP5J-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ATP5J (ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ATP5; ATP5A; ATPM; CF6; F6

Description (Vector)

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.

Gene ID

522

Gene Name (Vector)

ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6

Gene Symbol

ATP5J

HGNC ID

HGNC:847

ORF Size (aa)

327

RefSeq ID

NM_001685

RefSeq Synonyms

NM_001685, NM_001320267, NM_001320266, NM_001003703, NM_001003701, NM_001003697, NM_001003696, BC066310, BC001178,

Species

human

UniGene ID

Hs.246310

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.