human ATP5I shRNA silencing AAV

Name 
AAV-h-ATP5I-shRNA
Cat No 
shAAV-201727
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ATP5I.

shAAV-201727
AAV-h-ATP5I-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ATP5I (ATP synthase, H+ transporting, mitochondrial Fo complex subunit E). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATP synthase e chain, mitochondrial; ATP synthase membrane subunit e; ATP synthase subunit e, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex subunit E; ATP5I; ATP5K; ATPase subunit e; F1F0-ATP synthase, murine e subunit
Description (eCommerce)
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5I is the e subunit of the Fo complex [taken from NCBI Entrez Gene (Gene ID: 521)].
Description (Vector)
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.
Gene ID
521
Gene Name (eCommerce)
ATP synthase membrane subunit e
Gene Name (Vector)
ATP synthase, H+ transporting, mitochondrial Fo complex subunit E
Gene Symbol
ATP5I
HGNC ID
HGNC:846
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
210
Protein Name (eCommerce)
ATP synthase subunit e, mitochondrial
RefSeq ID
NM_007100
RefSeq Synonyms
NM_007100, BC105610, BC003679,
Research Areas
Cancer,Cardiology,Cell Biology,Metabolism/Metabolic Process,Mitochondrion,Transport
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,metabolism,cell_biology
Species
human
Target Sentence
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5I is the e subunit of the Fo complex [taken from NCBI Entrez Gene (Gene ID: 521)].
UniGene ID
Hs.85539
UniProt ID (eCommerce)
P56385

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.