human ATP5F1 shRNA silencing AAV

Name 
AAV-h-ATP5F1-shRNA
Cat No 
shAAV-201719
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ATP5F1.

shAAV-201719
AAV-h-ATP5F1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ATP5F1 (ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATP synthase B chain, mitochondrial; ATP synthase F(0) complex subunit B1, mitochondrial; ATP synthase peripheral stalk-membrane subunit b; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b; ATP synthase subunit b, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1; ATP5F1; ATPase subunit b; cell proliferation-inducing protein 47; H+-ATP synthase subunit b; PIG47
Description (eCommerce)
ATP5F1 is the b subunit of the proton channel complex of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) [taken from NCBI Entrez Gene (Gene ID: 515)].
Description (Vector)
ATP5F1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5F1 encodes the b subunit of the proton channel.
Gene ID
515
Gene Name (eCommerce)
ATP synthase peripheral stalk-membrane subunit b
Gene Name (Vector)
ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1
Gene Symbol
ATP5F1
HGNC ID
HGNC:840
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
771
ORF Size (bp)
771 bp
Protein Name (eCommerce)
ATP synthase F(0) complex subunit B1, mitochondrial
RefSeq ID
NM_001688
RefSeq Synonyms
NM_001688, BC016350, BC005960, BC005366,
Research Areas
Alzheimer's Disease,Cancer,Cardiology,Cell Biology,Huntington's Disease,Metabolism/Metabolic Process,Mitochondrion,Neurobiology,Neurodegeneration,Parkinson's Disease,Transport
Research Areas (Faceted)
cancer,neurobiology,cardiovascular,cell_biology,neurobiology,metabolism,cell_biology,neurobiology,cell_biology
Species
human
Target Sentence
ATP5F1 is the b subunit of the proton channel complex of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) [taken from NCBI Entrez Gene (Gene ID: 515)].
UniGene ID
Hs.514870
UniProt ID (eCommerce)
P24539

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.