human ASAP1 shRNA silencing AAV

Name 
AAV-h-ASAP1-shRNA
Cat No 
shAAV-201509
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ASAP1.

shAAV-201509
AAV-h-ASAP1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ASAP1 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; AMAP1; ARF GTPase-activating protein 1; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin, beta 4; CENTB4; DDEF1; DEF-1; development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; PAG2; PAP; PIP2-dependent ARF1 GAP; ZG14P
Description (eCommerce)
ASAP1 (Arf-Gap with SH3 domain, ANK repeat and PH domain-containing protein 1) is an Arf (ADP-ribosylation factor) GAP (GTPase-activating protein) that catalyzes the hydrolysis of GTP bound to Arf, a small GTP-binding protein. As a regulator of Arf activity ASAP1 has been shown to regulate cell adhesion and migration, and to be involved in carcinogenesis. Structurally, ASAP1 bears two ankyrin repeats, one Arf-GAP domain, one PH domain, one SH3 domain, and an N-terminal Bin, Amphiphysin and Rvs167 (BAR) domain.
Description (Vector)
ASAP1 encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. ASAP1 maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
50807
Gene Name (eCommerce)
ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Gene Name (Vector)
ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Gene Symbol
ASAP1
HGNC ID
HGNC:2720
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3390
ORF Size (bp)
3390 bp
Protein Name (eCommerce)
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
RefSeq ID
NM_018482
RefSeq Synonyms
NM_018482, NM_001247996, BC137135,
Research Areas
Cytoskeleton,EGFR Signaling,Immunology,RAC1 Signaling,Rho Signaling,Signal Transduction,T-cell Receptor Signaling,Zinc-finger
Research Areas (Faceted)
signal_transduction,cell_biology,immunology,cell_biology
Species
human
Target Sentence
ASAP1 (Arf-Gap with SH3 domain, ANK repeat and PH domain-containing protein 1) is an Arf (ADP-ribosylation factor) GAP (GTPase-activating protein) that catalyzes the hydrolysis of GTP bound to Arf, a small GTP-binding protein. As a regulator of Arf activity ASAP1 has been shown to regulate cell adhesion and migration, and to be involved in carcinogenesis. Structurally, ASAP1 bears two ankyrin repeats, one Arf-GAP domain, one PH domain, one SH3 domain, and an N-terminal Bin, Amphiphysin and Rvs167 (BAR) domain.
UniGene ID
Hs.655552
UniProt ID (eCommerce)
Q9ULH1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.