human ASAH2 shRNA silencing AAV

Name 
AAV-h-ASAH2-shRNA
Cat No 
shAAV-201507
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ASAH2.

shAAV-201507
AAV-h-ASAH2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ASAH2 (N-acylsphingosine amidohydrolase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
BCDase; HNAC1; LCDase; N-CDase; NCDase
Description (Vector)
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 (PubMed 11328816)).(supplied by OMIM, Mar 2008)
Gene ID
56624
Gene Name (Vector)
N-acylsphingosine amidohydrolase 2
Gene Symbol
ASAH2
HGNC ID
HGNC:18860
ORF Size (aa)
2181
ORF Size (bp)
2181 bp
RefSeq ID
NM_019893
RefSeq Synonyms
NM_019893, NM_001143974, BC107105,
Species
human
UniGene ID
Hs.512645

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.