human ASAH1 shRNA silencing AAV

Name
AAV-h-ASAH1-shRNA

Cat No
shAAV-201505

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ASAH1.

shAAV-201505

AAV-h-ASAH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ASAH1 (N-acylsphingosine amidohydrolase 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AC; ACDase; ASAH; PHP; PHP32; SMAPME

Description (Vector)

ASAH1 encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.

Gene ID

427

Gene Name (Vector)

N-acylsphingosine amidohydrolase 1

Gene Symbol

ASAH1

HGNC ID

HGNC:735

ORF Size (aa)

1170

ORF Size (bp)

1170 bp

RefSeq ID

NM_004315

RefSeq Synonyms

NM_177924, NM_004315, NM_001127505, BC016828, BC016481,

Species

human

UniGene ID

Hs.633993

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.