human APRT shRNA silencing AAV

Name
AAV-h-APRT-shRNA

Cat No
shAAV-201225

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human APRT.

shAAV-201225

AAV-h-APRT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human APRT (adenine phosphoribosyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AMP; APRTD

Description (Vector)

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Gene ID

353

Gene Name (Vector)

adenine phosphoribosyltransferase

Gene Symbol

APRT

HGNC ID

HGNC:626

ORF Size (aa)

543

ORF Size (bp)

543 bp

RefSeq ID

NM_000485

RefSeq Synonyms

NM_001030018, NM_000485, BC107151, BC106894,

Species

human

UniGene ID

Hs.28914

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.