human APOBEC3A shRNA silencing AAV

Name
AAV-h-APOBEC3A-shRNA

Cat No
shAAV-201181

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human APOBEC3A.

shAAV-201181

AAV-h-APOBEC3A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human APOBEC3A (apolipoprotein B mRNA editing enzyme catalytic subunit 3A). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

A3A; ARP3; bK150C2.1; PHRBN

Description (Vector)

APOBEC3A is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.

Gene ID

200315

Gene Name (Vector)

apolipoprotein B mRNA editing enzyme catalytic subunit 3A

Gene Symbol

APOBEC3A

HGNC ID

HGNC:17343

ORF Size (aa)

546

ORF Size (bp)

546 bp

RefSeq ID

NM_145699

RefSeq Synonyms

NM_145699, NM_001270406, BC144146, BC126416,

Species

human

UniGene ID

Hs.348983

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.