human APH1A shRNA silencing AAV

Name
AAV-h-APH1A-shRNA

Cat No
shAAV-201143

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human APH1A.

shAAV-201143

AAV-h-APH1A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human APH1A (aph-1 homolog A, gamma-secretase subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

6530402N02Rik; APH-1; APH-1A; CGI-78

Description (Vector)

APH1A encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants.

Gene ID

51107

Gene Name (Vector)

aph-1 homolog A, gamma-secretase subunit

Gene Symbol

APH1A

HGNC ID

HGNC:29509

ORF Size (aa)

798

ORF Size (bp)

798 bp

RefSeq ID

NM_016022

RefSeq Synonyms

NM_016022, NM_001243772, NM_001243771, NM_001077628, BC020590, BC017699, BC015568, BC009501, BC008732, BC001230,

Species

human

UniGene ID

Hs.108408

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.