human ACER1 shRNA silencing AAV

Name
AAV-h-ACER1-shRNA

Cat No
shAAV-200185

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ACER1.

shAAV-200185

AAV-h-ACER1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACER1 (alkaline ceramidase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ALKCDase1; ASAH3

Description (Vector)

Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 (PubMed 16477081); Sun et al., 2008 (PubMed 17713573)).(supplied by OMIM, Jul 2010)

Gene ID

125981

Gene Name (Vector)

alkaline ceramidase 1

Gene Symbol

ACER1

HGNC ID

HGNC:18356

ORF Size (aa)

795

ORF Size (bp)

795 bp

RefSeq ID

NM_133492

RefSeq Synonyms

NM_133492, BC112124, BC112122,

Species

human

UniGene ID

Hs.352609

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.