human ACADSB shRNA silencing AAV

Name
AAV-h-ACADSB-shRNA

Cat No
shAAV-200158

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ACADSB.

shAAV-200158

AAV-h-ACADSB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACADSB (acyl-CoA dehydrogenase, short/branched chain). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2-MEBCAD; ACAD7; SBCAD

Description (Vector)

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa.

Gene ID

Gene Name (Vector)

acyl-CoA dehydrogenase, short/branched chain

Gene Symbol

ACADSB

HGNC ID

HGNC:91

ORF Size (aa)

1299

ORF Size (bp)

1299 bp

RefSeq ID

NM_001609

RefSeq Synonyms

NM_001609, NM_001330174, BC013756,

Species

human

UniGene ID

Hs.81934

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.