mouse TOP3A Adenovirus

Name 
Ad-m-TOP3A
Cat No 
ADV-281173
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Mouse Top3a.

ADV-281173
Ad-m-TOP3A

Ready-to-use Adenovirus expressing Mouse Top3a (topoisomerase (DNA) III alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
Gene ID
21975
Gene Name (Vector)
topoisomerase (DNA) III alpha
Gene Symbol
Top3a
ORF Size (aa)
3012
ORF Size (bp)
3012 bp
RefSeq ID
NM_009410
RefSeq Synonyms
NM_009410, BC099689,
Species
mouse
UniGene ID
Mm.477819

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.