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mouse SMUG1 Adenovirus

Name 
Ad-m-SMUG1
Cat No 
ADV-272686
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Mouse Smug1.

ADV-272686
Ad-m-SMUG1

Ready-to-use Adenovirus expressing Mouse Smug1 (single-strand selective monofunctional uracil DNA glycosylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1200013B09Rik; A930006H09Rik; C85220
Description (Vector)
Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA.
Gene ID
71726
Gene Name (Vector)
single-strand selective monofunctional uracil DNA glycosylase
Gene Symbol
Smug1
ORF Size (aa)
840
RefSeq ID
NM_027885
RefSeq Synonyms
NM_027885, BC062960, BC050253,
Species
mouse
UniGene ID
Mm.463445

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.