mouse PIWIL2 Adenovirus
This is an Adenovirus expressing Mouse Piwil2.
ADV-268718
Ad-m-PIWIL2
Ready-to-use Adenovirus expressing Mouse Piwil2 (piwi-like RNA-mediated gene silencing 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
mili; Piwil1l
Description (Vector)
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity).
Gene ID
57746
Gene Name (Vector)
piwi-like RNA-mediated gene silencing 2
Gene Symbol
Piwil2
ORF Size (aa)
2916
ORF Size (bp)
2916 bp
RefSeq ID
NM_021308
RefSeq Synonyms
NM_021308, BC145717, BC138444,
Species
mouse
UniGene ID
Mm.85253
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.