human SF3A1 Adenovirus

Name 
Ad-h-SF3A1
Cat No 
ADV-222755
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human SF3A1.

ADV-222755
Ad-h-SF3A1

Ready-to-use Adenovirus expressing Human SF3A1 (splicing factor 3a subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
pre-mRNA processing 21; pre-mRNA splicing factor SF3a (120 kDa subunit); PRP21; PRPF21; SAP 114; SAP114; SF3A120; spliceosome-associated protein 114; splicing factor 3 subunit 1; splicing factor 3A subunit 1; splicing factor 3a, subunit 1, 120kD; splicing factor 3a, subunit 1, 120kDa
Description (eCommerce)
SF3a120/SAP114 is a 120 kDa subunit of the heterotrimeric splicing factor 3a (SF3A) protein complex. SF3A along with SF3B and a 12S RNA subunit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. SF3a120/SAP114 bears two SURP motif repeats and one ubiquitin-like domain. SURP motifs are found among proteins involved in regulated mRNA splicing. The SURP motifs along with the ubiquitin-like domain of SF3a120/SAP114 appear to function in the heterotrimeric formation between the 120, 60 and 66 kDa subunits.
Description (Vector)
SF3A1 encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID
10291
Gene Name (eCommerce)
splicing factor 3a subunit 1
Gene Name (Vector)
splicing factor 3a subunit 1
Gene Symbol
SF3A1
HGNC ID
HGNC:10765
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2382
ORF Size (bp)
2382 bp
Protein Name (eCommerce)
Splicing factor 3A subunit 1
RefSeq ID
NM_005877
RefSeq Synonyms
NM_005877, NM_001005409, BC007684, BC001976,
Research Areas
Gene Expression,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
SF3a120/SAP114 is a 120 kDa subunit of the heterotrimeric splicing factor 3a (SF3A) protein complex. SF3A along with SF3B and a 12S RNA subunit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. SF3a120/SAP114 bears two SURP motif repeats and one ubiquitin-like domain. SURP motifs are found among proteins involved in regulated mRNA splicing. The SURP motifs along with the ubiquitin-like domain of SF3a120/SAP114 appear to function in the heterotrimeric formation between the 120, 60 and 66 kDa subunits.
UniGene ID
Hs.406277
UniProt ID (eCommerce)
Q15459

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.