human RNASEN Adenovirus

Name 
Ad-h-RNASEN
Cat No 
ADV-221315
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human RNASEN.

ADV-221315
Ad-h-RNASEN

Ready-to-use Adenovirus expressing Human DROSHA (drosha ribonuclease III). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
drosha, double-stranded RNA-specific endoribonuclease; ETOHI2; HSA242976; nuclear RNase III Drosha; p241; Protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; RANSE3L; ribonuclease 3; Ribonuclease III; ribonuclease type III, nuclear; RN3; RNase III; RNASE3L; RNASEN
Description (eCommerce)
Drosha is one of three human RNase III enzymes (Drosha, Dicer, and Argonaute) that play a diverse role in the processing and maturation of RNA. Drosha, along with DGCR8 (DeGeorge Syndrome Chromomsomal Region 8, also known as Pasha in C. elegans and D. melanogaster) is a component of the Microprocessor complex. In this complex, Drosha functions as a double-stranded endoribonuclease critical to the first step in the processing of human microRNAs. During this step, Drosha is responsible for cleaving pri-miRNA to yield hairpin-shaped pre-miRNA for futher processing by the cytoplasmic RNase III enzyme, Dicer.
Description (Vector)
Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 (PubMed 12191433)). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 (PubMed 14508493)).(supplied by OMIM, Mar 2008)
Gene ID
29102
Gene Name (eCommerce)
drosha ribonuclease III
Gene Name (Vector)
drosha ribonuclease III
Gene Symbol
DROSHA
HGNC ID
HGNC:17904
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3579
ORF Size (bp)
3579 bp
Protein Name (eCommerce)
Ribonuclease 3
RefSeq ID
NM_013235
RefSeq Synonyms
NM_013235, NM_001100412, BC054003,
Research Areas
Cancer,Gene Expression,MicroRNAs in Cancer,RNA Binding,RNA Processing
Research Areas (Faceted)
cancer,cell_biology,genetics,transcription_translation
Species
human
Target Sentence
Drosha is one of three human RNase III enzymes (Drosha, Dicer, and Argonaute) that play a diverse role in the processing and maturation of RNA. Drosha, along with DGCR8 (DeGeorge Syndrome Chromomsomal Region 8, also known as Pasha in C. elegans and D. melanogaster) is a component of the Microprocessor complex. In this complex, Drosha functions as a double-stranded endoribonuclease critical to the first step in the processing of human microRNAs. During this step, Drosha is responsible for cleaving pri-miRNA to yield hairpin-shaped pre-miRNA for futher processing by the cytoplasmic RNase III enzyme, Dicer.
UniGene ID
Hs.97997
UniProt ID (eCommerce)
Q9NRR4

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.