human RAG2 Adenovirus

Name
Ad-h-RAG2

Cat No
ADV-220673

Availability
4-5 weeks

This is an Adenovirus expressing Human RAG2.

ADV-220673

Ad-h-RAG2

Ready-to-use Adenovirus expressing Human RAG2 (recombination activating 2). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

CMV

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

RAG-2

Description (Vector)

RAG2 encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Gene ID

5897

Gene Name (Vector)

recombination activating 2

Gene Symbol

RAG2

HGNC ID

HGNC:9832

ORF Size (aa)

1584

ORF Size (bp)

1584 bp

RefSeq ID

NM_000536

RefSeq Synonyms

NM_001243786, NM_001243785, NM_000536, BC022397,

Species

human

UniGene ID

Hs.714519

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.