human NAGPA Adenovirus
This is an Adenovirus expressing Human NAGPA.
ADV-216292
Ad-h-NAGPA
Ready-to-use Adenovirus expressing Human NAGPA (N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
APAA; UCE
Description (Vector)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.
Gene ID
51172
Gene Name (Vector)
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Gene Symbol
NAGPA
HGNC ID
HGNC:17378
ORF Size (aa)
930
ORF Size (bp)
930 bp
RefSeq ID
NM_016256
RefSeq Synonyms
NM_016256, BC012194,
Species
human
UniGene ID
Hs.21334
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.