human MCM4 Adenovirus

Name 
Ad-h-MCM4
Cat No 
ADV-215185
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human MCM4.

ADV-215185
Ad-h-MCM4

Ready-to-use Adenovirus expressing Human MCM4 (minichromosome maintenance complex component 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CDC21; CDC21 homolog; CDC54; DNA replication licensing factor MCM4; hCdc21; homolog of S. pombe cell devision cycle 21; IMD54; minichromosome maintenance deficient 4; NKCD; NKGCD; P1-CDC21
Description (eCommerce)
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding.
Description (Vector)
MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Gene ID
4173
Gene Name (eCommerce)
minichromosome maintenance complex component 4
Gene Name (Vector)
minichromosome maintenance complex component 4
Gene Symbol
MCM4
HGNC ID
HGNC:6947
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2592
ORF Size (bp)
2592 bp
Protein Name (eCommerce)
DNA replication licensing factor MCM4
RefSeq ID
NM_005914
RefSeq Synonyms
NM_182746, NM_005914, BC031061,
Research Areas
Cancer,Cell Cycle,DNA Replication,Helicase Activity,Replication,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding.
UniGene ID
Hs.460184
UniProt ID (eCommerce)
P33991

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.