human DDB1 Adenovirus

Name
Ad-h-DDB1

Cat No
ADV-206693

Availability
4-5 weeks

This is an Adenovirus expressing Human DDB1.

ADV-206693

Ad-h-DDB1

Ready-to-use Adenovirus expressing Human DDB1 (damage specific DNA binding protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

CMV

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; DDB p127 subunit; DDBA; DNA damage-binding protein 1; DNA damage-binding protein a; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; UV-DDB1; XAP1; XAP-1; xeroderma pigmentosum group E-complementing protein; XPCE; XPE; XPE-BF; XPE-binding factor

Description (eCommerce)

DDB1 (DNA Damage Binding Protein 1) is a subunit of the DNA damage-binding protein complex (DDB) involved in DNA repair. As part of a complex with Cul4-based ubiquitin ligase, Cul4-Ddb1 can directly modify chromatin through the ubiquitination of histones as well as ubiquitinate other chromatin bound factors. A deficiency in Cul4-Ddb1 ubiquitin ligase has been associated with xeroderma pigmentosum, a heritable disease characterized by defects in nucleotide excision repair and skin cancer development.

Description (Vector)

DDB1 is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. DDB1 also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.

Gene ID

1642

Gene Name (eCommerce)

damage specific DNA binding protein 1

Gene Name (Vector)

damage specific DNA binding protein 1

Gene Symbol

DDB1

HGNC ID

HGNC:2717

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

3423

ORF Size (bp)

3423 bp

Protein Name (eCommerce)

DNA damage-binding protein 1

RefSeq ID

NM_001923

RefSeq Synonyms

NM_001923, BC051764, BC050530, BC011686,

Research Areas

Cancer,Cell Cycle,DNA Damage/Repair,Host-Virus Interactions,Signal Transduction,Ubiquitination,Viral Carcinogenesis,Wnt Signaling

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,genetics,infectious_disease,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.705536

UniProt ID (eCommerce)

Q16531

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.