human BRAF Adenovirus

Name 
Ad-h-BRAF
Cat No 
ADV-202247
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human BRAF.

ADV-202247
Ad-h-BRAF

Ready-to-use Adenovirus expressing Human BRAF (B-Raf proto-oncogene, serine/threonine kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
94 kDa B-raf protein; B-raf; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; BRAF1; B-RAF1; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1
Description (eCommerce)
B-Raf proto-oncogene serine/threonine-protein kinase (BRAF) is a member of the raf/mil family of serine/threonine kinases. BRAF is involved in transducing mitogenic signals via the MAP kinase/ERKs signaling pathway. Mutations in BRAF are involved in a wide range of cancers such as lung, non-Hodgkin lymphoma, and colorectal cancer. Defects in BRAF are also the cause of cardiofaciocutaneous syndrome (CFC), an autosomal dominant syndrome characterized by facial anomalies, heart defects, and ectodermal abnormalities.
Description (Vector)
BRAF encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Gene ID
673
Gene Name (eCommerce)
B-Raf proto-oncogene, serine/threonine kinase
Gene Name (Vector)
B-Raf proto-oncogene, serine/threonine kinase
Gene Symbol
BRAF
HGNC ID
HGNC:1097
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2301
ORF Size (bp)
2301 bp
Protein Name (eCommerce)
Serine/threonine-protein kinase B-raf
RefSeq ID
NM_004333
RefSeq Synonyms
NM_004333, BC112079, BC101757,
Research Areas
Apoptosis,Autophagy,B-cell Receptor Signaling,Bladder Cancer,Breast Cancer,Cancer,Cardiology,Cardiomyopathy,Cardiovascular,Colorectal Cancer,Cytoskeleton,EGFR Signaling,EPO Signaling,Estrogen Signaling,FGF Signaling,Gene Expression,Glioma,GPCR Signaling,Host-Virus Interactions,Immunology,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kidney Cancer,Kinase/Phosphatase,Leukemia,Lung Cancer,MAPK Signaling,mTOR Signaling,Neurobiology,Neurodevelopment,NGF Signaling,PDGF Signaling,Prostate Cancer,Signal Transduction,T-cell Receptor Signaling,TSH Signaling,VEGF Signaling,Zinc-finger
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,immunology,infectious_disease,neurobiology,signal_transduction
Species
human
Target Sentence
B-Raf proto-oncogene serine/threonine-protein kinase (BRAF) is a member of the raf/mil family of serine/threonine kinases. BRAF is involved in transducing mitogenic signals via the MAP kinase/ERKs signaling pathway. Mutations in BRAF are involved in a wide range of cancers such as lung, non-Hodgkin lymphoma, and colorectal cancer. Defects in BRAF are also the cause of cardiofaciocutaneous syndrome (CFC), an autosomal dominant syndrome characterized by facial anomalies, heart defects, and ectodermal abnormalities.
UniGene ID
Hs.550061
UniProt ID (eCommerce)
P15056

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.